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Mandal, Guru Prasad
- Effects of Different Vegetable Oils on Rumen Fermentation and Conjugated Linoleic Acid Concentration in vitro
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Materials and Methods: Six vegetable oils including sunflower, soybean, sesame, rice bran, groundnut, and mustard oils were used at three dose levels (0%, 3% and 4% of substrate dry matter [DM] basis) in three replicates for each treatment in a completely randomized design using 6×3 factorial arrangement. Rumen fluid for microbial culture was collected from four goats fed on a diet of concentrate mixture and berseem hay at a ratio of 60:40 on DM basis. The in vitro fermentation was performed in 100 ml conical flakes containing 50 ml of culture media and 0.5 g of substrates containing 0%, 3% and 4% vegetable oils.
Results: Oils supplementation did not affect (p>0.05) in vitro DM digestibility, and concentrations of total volatile FAs and ammonia-N. Sunflower oil and soybean oil decreased (p<0.05) protozoal numbers with increasing levels of oils. Other oils had less pronounced effect (p>0.05) on protozoal numbers. Both trans-11 C18:1 FA and cis-9, trans-11 CLA concentrations were increased (p<0.05) by sunflower and soybean oil supplementation at 4% level with the highest concentration observed for sunflower oil. The addition of other oils did not significantly (p>0.05) increase the trans-11 C18:1 FA and cis-9, trans-11 CLA concentrations as compared to the control. The concentrations of stearic, oleic, linoleic, and linolenic acids were not altered (p>0.05) due to the addition of any vegetable oils.
Conclusion: Supplementation of sunflower and soybean oils enhanced beneficial trans-11 C18:1 FA and cis-9, trans-11 CLA concentrations in rumen fluid, while sesame, rice bran, groundnut, and mustard oils were ineffective in this study.
Authors
Affiliations
1 Department of Animal Nutrition, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata-700037, West Bengal, IN
1 Department of Animal Nutrition, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata-700037, West Bengal, IN
Source
Veterinary World, Vol 10, No 1 (2017), Pagination: 11-16Abstract
Aim: The objective of this study was to investigate the effect of different vegetable oils on rumen fermentation and concentrations of beneficial cis-9 trans-11 C18:2 conjugated linoleic acid (CLA) and trans-11 C18:1 fatty acid (FA) in the rumen fluid in an in vitro condition.Materials and Methods: Six vegetable oils including sunflower, soybean, sesame, rice bran, groundnut, and mustard oils were used at three dose levels (0%, 3% and 4% of substrate dry matter [DM] basis) in three replicates for each treatment in a completely randomized design using 6×3 factorial arrangement. Rumen fluid for microbial culture was collected from four goats fed on a diet of concentrate mixture and berseem hay at a ratio of 60:40 on DM basis. The in vitro fermentation was performed in 100 ml conical flakes containing 50 ml of culture media and 0.5 g of substrates containing 0%, 3% and 4% vegetable oils.
Results: Oils supplementation did not affect (p>0.05) in vitro DM digestibility, and concentrations of total volatile FAs and ammonia-N. Sunflower oil and soybean oil decreased (p<0.05) protozoal numbers with increasing levels of oils. Other oils had less pronounced effect (p>0.05) on protozoal numbers. Both trans-11 C18:1 FA and cis-9, trans-11 CLA concentrations were increased (p<0.05) by sunflower and soybean oil supplementation at 4% level with the highest concentration observed for sunflower oil. The addition of other oils did not significantly (p>0.05) increase the trans-11 C18:1 FA and cis-9, trans-11 CLA concentrations as compared to the control. The concentrations of stearic, oleic, linoleic, and linolenic acids were not altered (p>0.05) due to the addition of any vegetable oils.
Conclusion: Supplementation of sunflower and soybean oils enhanced beneficial trans-11 C18:1 FA and cis-9, trans-11 CLA concentrations in rumen fluid, while sesame, rice bran, groundnut, and mustard oils were ineffective in this study.
Keywords
Conjugated Linoleic Acid, Goat, Rumen Fluid, Vaccenic Acid, Vegetable Oil.Full Text
References
- Daley, C.A., Abbott, A., Doyle, P.S., Nader, G.A. and Larson, S. (2010) A review of fatty acid profiles and antioxidant content in grass-fed and grain-fed beef. Nutr. J., 9: 10. DOI: 10.1186/1475-2891-9-10.
- Patra, A.K. (2014a) Exploring the benefits of feeding tannin containing diets for enhancing the nutritional values of milk and meat of ruminants. Indian J. Anim. Health, 53: 63-76.
- Mandal, G.P., Roy, A. and Patra, A.K. (2014) Effects of feeding plant additives rich in saponins and essential oils on the performance, carcass traits and conjugated linoleic acid concentrations in muscle and adipose tissues of Black Bengal goats. Anim. Feed Sci. Technol., 197: 76-84.
- Mandal, G.P., Roy, A. and Patra, A.K. (2016) Effects of plant extracts rich in tannins, saponins and essential oils on rumen fermentation and conjugated linoleic acid conentrations in vitro. Indian J. Anim. Health, 55: 49-60.
- Benjamin, S. and Spener, F. (2009) Conjugated linoleic acids as functional food: An insight into their health benefits. Nutr. Metab. (Lond.), 6: 36. DOI: 10.1186/1743-7075-6-36.
- Field, C.J., Blewett, H.H., Proctor, S. and Vine, D. (2009) Human health benefits of vaccenic acid. Appl. Physiol. Nutr. Metab., 34: 979-991.
- Wang, Y., Jacome-Sosa, M.M. and Proctor, S.D. (2012) The role of ruminant trans fat as a potential nutraceutical in the prevention of cardiovascular disease. Food Res. Int., 46: 460-468.
- Chin, S.F., Liu, W., Storkson, J.M., Ha, Y.L. and Pariza, W.M. (1992) Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J. Food Compost. Anal., 5: 185-197.
- Lock, A.L. and Bauman, D.E. (2004) Modifying milk fat composition of dairy cows to enhance fatty acids beneficial to human health. Lipids, 39: 1197-1206.
- Shingfield, K.J., Bonnet, M. and Scollan, N.D. (2013) Recent developments in altering the fatty acid composition of ruminant-derived foods. Anim. Suppl., 1: 132-162.
- Griinari, J.M., Corl, B.A., Lacy, S.H., Chouinard, P.Y., Nurmela, K.V.V. and Bauman, D.E. (2000) Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Δ9-desaturase. J. Nutr., 130: 2285-2291.
- Mosley, E.E., Powell, G.L., Riley, M.B. and Jenkins, T.C. (2002) Microbial biohydrogenation of oleic acid to trans isomers in vitro. J. Lipid Res., 43: 290-296.
- Patra, A.K. (2014b) A meta-analysis of the effect of dietary fat on enteric methane production, digestibility and rumen fermentation in sheep, and a comparison of these responses between cattle and sheep. Livest. Sci., 162: 97-103.
- Menke, K.H. and Steingass, H. (1988) Estimation of the energetic feed value from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev., 28: 7-55.
- Patra, A.K., Stiverson, J. and Yu, Z. (2012) Effects of quillaja and yucca saponins on communities and select populations of rumen bacteria and archaea, and fermentation in vitro. J. Appl. Microbiol., 113: 1329-1340.
- Kamra, D.N., Sawal, R.K., Pathak, N.N., Kewalramani, N. and Agarwal, N. (1991) Diurnal variation in ciliate protozoa in the rumen of blackbuck (Antilope cervicapra). Lett. Appl. Microbiol., 13: 165-167.
- Barnett, J.G.A. and Reid, R.L. (1956) Studies on the production of volatile fatty acids from the grass by rumen liquor in an artificial rumen. J. Agric. Sci., 48: 315-321.
- AOAC. (2005) Official Methods of Analysis. 18th ed. Association of Official Analytical Chemists, Washington, DC.
- O'Fallon, J.V., Busboom, J.R., Nelson, M.L. and Gaskins, C.T. (2007) A direct method for fatty acid methyl ester synthesis: Application to wet meat tissues, oils, and feedstuffs. J. Anim. Sci., 85: 1511-1521.
- SPSS. (1996) Statistical Packages for Social Sciences. Version 7.5. SPSS Inc., USA, IL.
- Patra, A.K. and Yu, Z. (2013) Effects of coconut and fish oils on ruminal methanogenesis, fermentation, and abundance and diversity of microbial populations in vitro. J. Dairy Sci., 96: 1782-1792.
- Patra, A.K. (2013) The effect of dietary fats on methane emissions, and its other effects on digestibility, rumen fermentation and lactation performance in cattle: A meta-analysis. Livest. Sci., 155: 244-254.
- Gomez-Cortes, P., Frutos, P., Mantecon, A.R., Juarez, M., de la Fuente, M.A. and Hervas, G. (2008) Milk production, conjugated linoleic acid content, and in vitro ruminal fermentation in response to high levels of soybean oil in dairy ewe diet. J. Dairy Sci., 91: 1560-1569.
- El-Sherbiny M., Cieslak, A., Pers-Kamczyc, E., Szczechowiak, J., Kowalczyk, D. and Szumacher-Strabel, M. (2016) Short communication: A nanoemulsified form of oil blends positively affects the fatty acid proportion in ruminal batch cultures. J. Dairy Sci., 99: 399-407.
- Harfoot, C.G. and Hazelwood, G.P. (1997) Lipid metabolism in the rumen. In: Hobson, P.N., editor. The Rumen Microbial Ecosystem. Elsevier, New York, NY. p382-426.
- Szczechowiaka, J., Szumacher-Strabel, M., El-Sherbiny, M., Pers-Kamczy, E., Pawlak, P. and Cieslak, A. (2016) Rumen fermentation, methane concentration and fatty acid proportion in the rumen and milk of dairy cows fed condensed tannin and/or fish-soybean oils blend. Anim. Feed Sci. Technol., 216: 93-107.
- Boles, J.A., Kott, R.W., Hatfield, P.G., Bergman, J.W. and Flynn, C.R. (2005) Supplemental safflower oil affects the fatty acid profile, including conjugated linoleic acid, of lambs. J. Anim. Sci., 83: 2175-2181.
- Noci, F., French, P., Monahan, F.J. and Moloney, A.P. (2007) The fatty acid composition of muscle fat and subcutaneous adipose tissue of grazing heifers supplemented with plant oil-enriched concentrates. J. Anim. Sci., 85: 1062-1073.
- Roy, A., Mandal, G.P. and Patra, A.K. (2013) Evaluating the performance, carcass traits and conjugated linoleic acid content in muscle and adipose tissues of Black Bengal goats fed soybean oil and sunflower oil. Anim. Feed Sci. Technol., 185: 43-52.
- Li, X.Z., Yen, C.G., Lee, H.G., Choi, C.W. and Song, M.K. (2012) Influence of dietary plant oils on mammary lipogenic enzymes and the conjugated linoleic acid content of plasma and milk fat of lactating goats. Anim. Feed Sci. Technol., 174: 26-35.
- Lunsin, R., Wanapat, M., Yuangklang, C. and Rowlinson, P. (2012) Effect of rice bran oil supplementation on rumen fermentation, milk yield and milk composition in lactating dairy cows. Livest. Sci., 145: 167-173.
- Dai, X.J., Wang, C. and Zhu, Q. (2011) Milk performance of dairy cows supplemented with rapeseed oil, peanut oil and sunflower seed oil. Czech. J. Anim. Sci., 56: 181-191.